home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410p.zip
/
M94A3290.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
3KB
|
49 lines
Document 3290
DOCN M94A3290
TI HIV infection of adherent cells and expression of membrane molecules.
DT 9412
AU Dolei A; Serra C; Tilocca F; Ceraulo F; Conaldi PG; Modesti A; Ameglio
F; Toniolo A; Inst. of Microbiology & Virology, Univ. of Sassari, Italy.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):11 (abstract no. 017A). Unique
Identifier : AIDSLINE ICA10/94369285
AB OBJECTIVE: Human cells of mesenchymal and epithelial origin were
analyzed with respect to plasmamembrane molecules and sensitivity to HIV
adsorption and replication. METHODS: By the use of a panel of monoclonal
antibodies, we evaluated the expression and modulation by HIV infection,
of molecules involved in HIV adsorption, such as CD4, galactocerebroside
(GalC) and CD26, and molecules that can contribute to HIV unspecific
binding to cells, such as HLA Class I and II and several adhesion
molecules (VCAM, ICAM, ELAM, CD44, CD49d, etc.). HIV-1 adsorption and
replication (intra- and extracellular virus) was evaluated as ability to
form syncytia in the C8166 T cell line, and as production of p24 antigen
in ELISA. RESULTS: HIV-1 binds to and replicates in all the cells
tested. CD4 expression was detected in normal fibroblasts from lung,
breast and gingiva, and, as expected, in HeLa-T4 cells. GalC was
detected in mammary epithelial MEC-2 cells and in HeLa-T4, and, to a
lesser extent, on normal fibroblasts; rhabdomyosarcoma and endothelial
cells were GalC-negative. CD26 was found on all the cells. It was not
possible to correlate CD26 expression on cells to HIV binding
capability, or to syncytia formation. However, CD26 expression was
stimulated in HIV-infected cells. In addition, HIV infection of
DR-negative MEC-2 cells induced the expression of DR, and modulated that
of some adhesion molecules. It remains to clarify whether the effects
observed in infected cells are due to HIV per se or they are mediated by
some of the cytokines induced by HIV in adherent cells, such as IL-6,
TNF alpha and IL-1 beta, that have been found to increase CD4 expression
and HIV adsorption in fibroblasts and HeLa-T4 cells (Dolei et al., Arch
Virol 134:157, 1994). HIV infection of MEC-2 cells downregulated the
expression of some markers of mammary differentiation, such as EMA
(epithelial membrane antigen) and HMFG1 (human milk fat globulel). This
was in keeping with the reduction of lipid vacuoles formation observed
in infected monolayers scored under the microscope.
DE Adsorption Antigens, CD4/METABOLISM Antigens, Differentiation,
T-Lymphocyte/METABOLISM Cell Adhesion Molecules/METABOLISM Cells,
Cultured Galactosylceramides/METABOLISM Human HIV Core Protein
p24/BIOSYNTHESIS HIV Infections/*IMMUNOLOGY/MICROBIOLOGY HIV-1/GROWTH
& DEVELOPMENT HLA Antigens/METABOLISM Support, Non-U.S. Gov't Virus
Replication MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).